The herbicide 2,4-dichlorophenoxyacetic acid induces the generation of free-radicals and associated oxidative stress responses in yeast.
Identifieur interne : 000E41 ( Main/Exploration ); précédent : 000E40; suivant : 000E42The herbicide 2,4-dichlorophenoxyacetic acid induces the generation of free-radicals and associated oxidative stress responses in yeast.
Auteurs : Miguel C. Teixeira [Portugal] ; João P. Telo ; Nuno F. Duarte ; Isabel Sá-CorreiaSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2004.
Descripteurs français
- KwdFr :
- Acide 2,4-dichlorophénoxy-acétique (pharmacologie), Catalase (composition chimique), Catalase (métabolisme), Facteurs temps (MeSH), Glutarédoxines (MeSH), Glutathion (métabolisme), Glutathione transferase (métabolisme), Herbicides (pharmacologie), Mutation (MeSH), Oxidoreductases (composition chimique), Oxydants (composition chimique), Piégeage de spin (méthodes), Protéines de Saccharomyces cerevisiae (composition chimique), Radicaux libres (MeSH), Relation dose-effet des médicaments (MeSH), Saccharomyces cerevisiae (effets des médicaments et des substances chimiques), Saccharomyces cerevisiae (métabolisme), Spectroscopie de résonance de spin électronique (MeSH), Stress oxydatif (MeSH), Superoxide dismutase (composition chimique), Système acellulaire (MeSH).
- MESH :
- composition chimique : Catalase, Oxidoreductases, Oxydants, Protéines de Saccharomyces cerevisiae, Superoxide dismutase.
- effets des médicaments et des substances chimiques : Saccharomyces cerevisiae.
- métabolisme : Catalase, Glutathion, Glutathione transferase, Saccharomyces cerevisiae.
- méthodes : Piégeage de spin.
- pharmacologie : Acide 2,4-dichlorophénoxy-acétique, Herbicides.
- Facteurs temps, Glutarédoxines, Mutation, Radicaux libres, Relation dose-effet des médicaments, Spectroscopie de résonance de spin électronique, Stress oxydatif, Système acellulaire.
English descriptors
- KwdEn :
- 2,4-Dichlorophenoxyacetic Acid (pharmacology), Catalase (chemistry), Catalase (metabolism), Cell-Free System (MeSH), Dose-Response Relationship, Drug (MeSH), Electron Spin Resonance Spectroscopy (MeSH), Free Radicals (MeSH), Glutaredoxins (MeSH), Glutathione (metabolism), Glutathione Transferase (metabolism), Herbicides (pharmacology), Mutation (MeSH), Oxidants (chemistry), Oxidative Stress (MeSH), Oxidoreductases (chemistry), Saccharomyces cerevisiae (drug effects), Saccharomyces cerevisiae (metabolism), Saccharomyces cerevisiae Proteins (chemistry), Spin Trapping (methods), Superoxide Dismutase (chemistry), Time Factors (MeSH).
- MESH :
- chemical , chemistry : Catalase, Oxidants, Oxidoreductases, Saccharomyces cerevisiae Proteins, Superoxide Dismutase.
- chemical , metabolism : Catalase, Glutathione, Glutathione Transferase.
- chemical , pharmacology : 2,4-Dichlorophenoxyacetic Acid, Herbicides.
- drug effects : Saccharomyces cerevisiae.
- metabolism : Saccharomyces cerevisiae.
- methods : Spin Trapping.
- Cell-Free System, Dose-Response Relationship, Drug, Electron Spin Resonance Spectroscopy, Free Radicals, Glutaredoxins, Mutation, Oxidative Stress, Time Factors.
Abstract
The pro-oxidant action of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is demonstrated in this study using Saccharomyces cerevisiae as a eukaryotic experimental model. Evidence is presented for the generation of hydroxyl-radicals, in yeast cells suddenly exposed to 2,4-D, detected by in vivo electron paramagnetic resonance (EPR) spectroscopy using 5,5'-dimethyl-1-pyrroline N-oxide and 5-tert-butoxycarbonyl-5-methyl-1-pyrroline N-oxide as spin-traps. The intensity of the EPR spectra was dependent on the concentration of herbicide tested and was consistently higher in a mutant (Deltasod1) devoid of the cytosolic CuZn-superoxide dismutase. A time-course-dependent variation of the level of free-radical adducts was registered upon sudden exposure of an yeast cell population to concentrations of 2,4-D that lead to an initial period of viability loss, before resumption of inhibited growth by the viable adapted population. The variation pattern of the level of hydroxyl-radical adducts correlated with the one determined for the activity of Sod1p, cytosolic catalase Ctt1p, and the dithiol glutaredoxins Grx1p and Grx2p.
DOI: 10.1016/j.bbrc.2004.09.158
PubMed: 15485668
Affiliations:
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Teixeira</name><affiliation wicri:level="1"><nlm:affiliation>Biological Sciences Research Group, Centro de Engenharia Biológica e Quimica, Instituto Superior Técnico, 1049-001 Lisbon, Portugal.</nlm:affiliation><country xml:lang="fr">Portugal</country><wicri:regionArea>Biological Sciences Research Group, Centro de Engenharia Biológica e Quimica, Instituto Superior Técnico, 1049-001 Lisbon</wicri:regionArea><wicri:noRegion>1049-001 Lisbon</wicri:noRegion></affiliation></author><author><name sortKey="Telo, Joao P" sort="Telo, Joao P" uniqKey="Telo J" first="João P" last="Telo">João P. Telo</name></author><author><name sortKey="Duarte, Nuno F" sort="Duarte, Nuno F" uniqKey="Duarte N" first="Nuno F" last="Duarte">Nuno F. Duarte</name></author><author><name sortKey="Sa Correia, Isabel" sort="Sa Correia, Isabel" uniqKey="Sa Correia I" first="Isabel" last="Sá-Correia">Isabel Sá-Correia</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>2,4-Dichlorophenoxyacetic Acid (pharmacology)</term><term>Catalase (chemistry)</term><term>Catalase (metabolism)</term><term>Cell-Free System (MeSH)</term><term>Dose-Response Relationship, Drug (MeSH)</term><term>Electron Spin Resonance Spectroscopy (MeSH)</term><term>Free Radicals (MeSH)</term><term>Glutaredoxins (MeSH)</term><term>Glutathione (metabolism)</term><term>Glutathione Transferase (metabolism)</term><term>Herbicides (pharmacology)</term><term>Mutation (MeSH)</term><term>Oxidants (chemistry)</term><term>Oxidative Stress (MeSH)</term><term>Oxidoreductases (chemistry)</term><term>Saccharomyces cerevisiae (drug effects)</term><term>Saccharomyces cerevisiae (metabolism)</term><term>Saccharomyces cerevisiae Proteins (chemistry)</term><term>Spin Trapping (methods)</term><term>Superoxide Dismutase (chemistry)</term><term>Time Factors (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acide 2,4-dichlorophénoxy-acétique (pharmacologie)</term><term>Catalase (composition chimique)</term><term>Catalase (métabolisme)</term><term>Facteurs temps (MeSH)</term><term>Glutarédoxines (MeSH)</term><term>Glutathion (métabolisme)</term><term>Glutathione transferase (métabolisme)</term><term>Herbicides (pharmacologie)</term><term>Mutation (MeSH)</term><term>Oxidoreductases (composition chimique)</term><term>Oxydants (composition chimique)</term><term>Piégeage de spin (méthodes)</term><term>Protéines de Saccharomyces cerevisiae (composition chimique)</term><term>Radicaux libres (MeSH)</term><term>Relation dose-effet des médicaments (MeSH)</term><term>Saccharomyces cerevisiae (effets des médicaments et des substances chimiques)</term><term>Saccharomyces cerevisiae (métabolisme)</term><term>Spectroscopie de résonance de spin électronique (MeSH)</term><term>Stress oxydatif (MeSH)</term><term>Superoxide dismutase (composition chimique)</term><term>Système acellulaire (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Catalase</term><term>Oxidants</term><term>Oxidoreductases</term><term>Saccharomyces cerevisiae Proteins</term><term>Superoxide Dismutase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Catalase</term><term>Glutathione</term><term>Glutathione Transferase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>2,4-Dichlorophenoxyacetic Acid</term><term>Herbicides</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Catalase</term><term>Oxidoreductases</term><term>Oxydants</term><term>Protéines de Saccharomyces cerevisiae</term><term>Superoxide dismutase</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Spin Trapping</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Catalase</term><term>Glutathion</term><term>Glutathione transferase</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Piégeage de spin</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Acide 2,4-dichlorophénoxy-acétique</term><term>Herbicides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell-Free System</term><term>Dose-Response Relationship, Drug</term><term>Electron Spin Resonance Spectroscopy</term><term>Free Radicals</term><term>Glutaredoxins</term><term>Mutation</term><term>Oxidative Stress</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Facteurs temps</term><term>Glutarédoxines</term><term>Mutation</term><term>Radicaux libres</term><term>Relation dose-effet des médicaments</term><term>Spectroscopie de résonance de spin électronique</term><term>Stress oxydatif</term><term>Système acellulaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The pro-oxidant action of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is demonstrated in this study using Saccharomyces cerevisiae as a eukaryotic experimental model. Evidence is presented for the generation of hydroxyl-radicals, in yeast cells suddenly exposed to 2,4-D, detected by in vivo electron paramagnetic resonance (EPR) spectroscopy using 5,5'-dimethyl-1-pyrroline N-oxide and 5-tert-butoxycarbonyl-5-methyl-1-pyrroline N-oxide as spin-traps. The intensity of the EPR spectra was dependent on the concentration of herbicide tested and was consistently higher in a mutant (Deltasod1) devoid of the cytosolic CuZn-superoxide dismutase. A time-course-dependent variation of the level of free-radical adducts was registered upon sudden exposure of an yeast cell population to concentrations of 2,4-D that lead to an initial period of viability loss, before resumption of inhibited growth by the viable adapted population. The variation pattern of the level of hydroxyl-radical adducts correlated with the one determined for the activity of Sod1p, cytosolic catalase Ctt1p, and the dithiol glutaredoxins Grx1p and Grx2p.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15485668</PMID><DateCompleted><Year>2004</Year><Month>12</Month><Day>14</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0006-291X</ISSN><JournalIssue CitedMedium="Print"><Volume>324</Volume><Issue>3</Issue><PubDate><Year>2004</Year><Month>Nov</Month><Day>19</Day></PubDate></JournalIssue><Title>Biochemical and biophysical research communications</Title><ISOAbbreviation>Biochem Biophys Res Commun</ISOAbbreviation></Journal><ArticleTitle>The herbicide 2,4-dichlorophenoxyacetic acid induces the generation of free-radicals and associated oxidative stress responses in yeast.</ArticleTitle><Pagination><MedlinePgn>1101-7</MedlinePgn></Pagination><Abstract><AbstractText>The pro-oxidant action of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is demonstrated in this study using Saccharomyces cerevisiae as a eukaryotic experimental model. Evidence is presented for the generation of hydroxyl-radicals, in yeast cells suddenly exposed to 2,4-D, detected by in vivo electron paramagnetic resonance (EPR) spectroscopy using 5,5'-dimethyl-1-pyrroline N-oxide and 5-tert-butoxycarbonyl-5-methyl-1-pyrroline N-oxide as spin-traps. The intensity of the EPR spectra was dependent on the concentration of herbicide tested and was consistently higher in a mutant (Deltasod1) devoid of the cytosolic CuZn-superoxide dismutase. A time-course-dependent variation of the level of free-radical adducts was registered upon sudden exposure of an yeast cell population to concentrations of 2,4-D that lead to an initial period of viability loss, before resumption of inhibited growth by the viable adapted population. The variation pattern of the level of hydroxyl-radical adducts correlated with the one determined for the activity of Sod1p, cytosolic catalase Ctt1p, and the dithiol glutaredoxins Grx1p and Grx2p.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Teixeira</LastName><ForeName>Miguel C</ForeName><Initials>MC</Initials><AffiliationInfo><Affiliation>Biological Sciences Research Group, Centro de Engenharia Biológica e Quimica, Instituto Superior Técnico, 1049-001 Lisbon, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Telo</LastName><ForeName>João P</ForeName><Initials>JP</Initials></Author><Author ValidYN="Y"><LastName>Duarte</LastName><ForeName>Nuno F</ForeName><Initials>NF</Initials></Author><Author ValidYN="Y"><LastName>Sá-Correia</LastName><ForeName>Isabel</ForeName><Initials>I</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biochem Biophys Res Commun</MedlineTA><NlmUniqueID>0372516</NlmUniqueID><ISSNLinking>0006-291X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005609">Free Radicals</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D054477">Glutaredoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006540">Herbicides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016877">Oxidants</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029701">Saccharomyces cerevisiae Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>2577AQ9262</RegistryNumber><NameOfSubstance UI="D015084">2,4-Dichlorophenoxyacetic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.-</RegistryNumber><NameOfSubstance UI="D010088">Oxidoreductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.6</RegistryNumber><NameOfSubstance UI="D002374">Catalase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.15.1.1</RegistryNumber><NameOfSubstance UI="D013482">Superoxide Dismutase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.5.1.18</RegistryNumber><NameOfSubstance UI="D005982">Glutathione Transferase</NameOfSubstance></Chemical><Chemical><RegistryNumber>GAN16C9B8O</RegistryNumber><NameOfSubstance UI="D005978">Glutathione</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015084" MajorTopicYN="N">2,4-Dichlorophenoxyacetic Acid</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002374" MajorTopicYN="N">Catalase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002474" MajorTopicYN="N">Cell-Free System</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004305" MajorTopicYN="N">Dose-Response Relationship, Drug</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004578" MajorTopicYN="N">Electron Spin Resonance Spectroscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005609" MajorTopicYN="Y">Free Radicals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054477" MajorTopicYN="N">Glutaredoxins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005978" MajorTopicYN="N">Glutathione</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005982" MajorTopicYN="N">Glutathione Transferase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006540" MajorTopicYN="N">Herbicides</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016877" MajorTopicYN="N">Oxidants</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="Y">Oxidative Stress</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010088" MajorTopicYN="N">Oxidoreductases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012441" MajorTopicYN="N">Saccharomyces cerevisiae</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029701" MajorTopicYN="N">Saccharomyces cerevisiae Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019097" MajorTopicYN="N">Spin Trapping</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013482" MajorTopicYN="N">Superoxide Dismutase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time 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uniqKey="Duarte N" first="Nuno F" last="Duarte">Nuno F. Duarte</name><name sortKey="Sa Correia, Isabel" sort="Sa Correia, Isabel" uniqKey="Sa Correia I" first="Isabel" last="Sá-Correia">Isabel Sá-Correia</name><name sortKey="Telo, Joao P" sort="Telo, Joao P" uniqKey="Telo J" first="João P" last="Telo">João P. Telo</name></noCountry><country name="Portugal"><noRegion><name sortKey="Teixeira, Miguel C" sort="Teixeira, Miguel C" uniqKey="Teixeira M" first="Miguel C" last="Teixeira">Miguel C. Teixeira</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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